Mature leaves were dissected, fixed in FAA (ethanol, glacial acetic acid, and formaldehyde), dehydrated in ethanol series, and dried in carbon dioxide using a Denton DCP-1 critical point drying apparatus. Leaves were mounted on stubs, coated with 25 nm gold palladium using a Hummer II Sputter Coater, and examined using a Cambridge S-260 scanning electron microscope. Thicknesses of total leaf, upper and lower epidermis, mesophyll, and sponge tissues were measured with a light microscope equipped with a micrometer. The leaf thicknesses were monitored from April to October 2000 for all 35 species.
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