Measurements of leaf physiology and pigment composition are often highly variable leaf-to-leaf and over time, and limit the ability to delineate plant responses, specifically as a result of UV exposure. Variability was minimized by measuring only the central soybean leaflets of fully-expanded, sunlit (when possible) leaves at similar times during each day of the experiment. All plants in the greenhouse studies were well-watered throughout their development to minimize confounding water stress effects on the plant's physiological activity. However, variability in measurements remains due to other genetic differences in plants, variability in stomatal openings across the leaf, and environmental conditions, such as cloud cover or UV obstruction of direct radiation due to the UV light bank on a given day for a given leaf.
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