How To Grow Tobacco At Home
Using the pNPI106 vector, we could obtain marker-free transgenic tobacco plants from only 3 of 63 ESP lines (4.8 ) by 8 months after infection of Agrobacterium.2 Also, we obtained marker-free transgenic tobacco plants from 10 of 48 ESP lines by 4 months after infection using the pNPI132 vector. Finally, marker-free transgenic tobacco plants appeared from 32 of 48 ESP lines (67 ) by 8 months after infection.3 These results indicate that the MATVS could produce marker-free transgenic plants without sexual crossing and that the R RS-type MATVS is the more practical of the two (Table 25.1).
Pilon-Smits et al. (1995) demonstrated that the drought tolerance of tobacco plants could be improved by engineering the plants to produce increased fructan sugar levels. Tobacco (Nicotiana tabacum) plants engineered with the bacterial sacB gene, which encoded a fructosyl transferase enzyme targeted to the plant vacuole, exhibited a 55 increase in growth rate and a 59 increase in biomass production relative to wild-type control plants under water-deficit conditions. The researchers concluded that the introduction of fructans into non-fructan-producing species will potentially enhance resistance to drought stress. The ability to engineer production of the osmoprotectant mannitol in higher plants has been demonstrated in both Arabidopsis (Thomas et al., 1995) and tobacco (Tarczynski et al., 1993). Seeds from Arabidopsis plants overexpressing the bacterial mltd gene encoding mannitol 1-phosphate dehydrogenase exhibited enhanced germination rates in the presence of elevated salt...
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