Carbon monoxide 2 N03 5 CO H20 N2 2 OH 5 C02

Denitrification can also be accomplished by autotrophic bacteria, which can use hydrogen or various reduced-sulphur compounds as energy sources. Under autotrophic growth conditions, no organic carbon sources are required, rather carbon dioxide or bicarbonate is used as a carbon source for cell synthesis.

Paracocus denitrificans and Thiobacillus denitrificans can denitrify using hydrogen and reduced-sulphur compounds, respectively. Both of these bacilli can also grow heterotrophically, if an organic carbon source is present.

The following stoichiometric relationships for hydrogen and sulphur have been reported:

Hydrogen (Kurt et al. 1987):

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