The Proteomic Approach

This approach, a joint venture with the group of Dr Michael Djordjevic at the Australian National University, has examined the protein profiles for cells of WSM419 grown at pH 7.0 and exposed to pH 5.7 either transiently or long-term. The gels detected some 52 proteins whose expressions are changed by low pH exposure; of those identified by micro-sequencing, 9 were down-regulated and 7

up-regulated. There was no overlap between the group of proteins identified from proteome analysis and those predicted from the genes whose insertional fusions were induced by low pH.

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