N. Ausmees, M. Lindberg
Dept of Microbiology, Swedish University of Agricultural Sciences, SLU, Uppsala, Sweden 1. Introduction
Using phage display technique we identified a family of secreted proteins in R. leguminosarum, which interact with the bacterial cell surface (Ausmees et al. 2001). These proteins (RapAl, -A2, -A3, RapB and RapC) and some others (PlyA and PlyB, Finnie et al. 1998) contain one or several so-called Ra-domains, which are responsible for this binding (Figure 1). Here we show that the Ra-domain specifically recognizes a carbohydrate epitope of EPS and CPS of i?, leguminosarum.
PlyA PlyB Endoglucanase activity, heptapeptide repeats RapAl, -A2, -A3 At least three alleles in the genome
RapB _Similarity to an endoglucanase
'WTH'* sequence not available, size -60 kDa
Figure 1. Domain architecture of proteins containing Ra-domains (boxes).
Was this article helpful?