Under low P supply three different clones encoding for asparagine synthetase LJAS2 have been down-regulated significantly. In earlier studies an accumulation of asparagine under P deficiency has been observed (Steward, Larher 1980; Almeida et al. 2000). Repression by nitrogen-transport amino acids of the expression of the asparagine synthetase gene ASN2 in Arabidopsis has been shown by Lam et al. 1998 and Wang et al. 2000. Therefore, we suggest a feedback mechanism of asparagine on the expression of asparagine synthetase.
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