Results and Discussion

The Nod" selections showed poor root hair deformation except for ICCV2M (equal to its parent line). Cortical cell divisions were completely absent. The phenotypes suggested that the nodulation process is probably blocked at the early stages.

ENOD40 from Sesbania and ENOD12 from pea were used as probes for chickpea for Southern and Northern hybridizations but the heterologous probes gave faint signals. RT-PCR was performed on total RNA of nodulation variants to study the expression of ENOD40, ENOD2 and ENOD12 using primers designed from soybean and pea. They were also amplified by PCR from chickpea genomic DNA to be used as homologous probes. The expression pattern of these genes in nodulation variants ICC4918M and ICCV2M and their normal parent lines were compared. ENOD40 and ENOD12 were expressed both in normal and non-nodulating chickpea lines.

The strategy of differential display of mRNA by PCR (DDRT-PCR) is being adopted to analyze the expression of nodulation related genes.

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