Procedures

One mutant plant individual of Medicago sativa MN-1008 was crossed with a Nod alfalfa individual {Medicago sativa cv. Nagyszenasi) to generate the F1 plants which were grown in pots and self-mated to produce the F2 progenies. Individual plants from selected F2 families were used as mapping population to determine the map position of the non-nodulation trait and linked molecular markers. Plant nodulation test, DNA isolation, PCR and RFLP mapping were performed as described by Endre et al. (1996) and Kalo et al. (2000). Genetic analysis of the data was performed as described (Kalo et al. 2000; Kiss et al. 1998).

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