Sequence analysis was performed using BLAST programs. regR and regM were mutated with the insertion of a kanamycin cassette within the gene and introduced into the genome using a suicide vector (Quandt, Hynes 1993). Mutant phenotype was analyzed using competition, motility, osmolarity, biofilm, and nodulation assays. To identify genes regulated by RegR, the mutant background was mutagenized with mTnSSSgmvl^O (Wilson et al. 1995). This created random promoter-gw^ fusions within the genome. Wild-type regR was introduced in trans on a broad host range plasmid. Differential gusA expression in complemented vs. uncomplemented with wild-type regR suggests a role in transcriptional regulation.
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