NifDK

Lanes:

1.

Complete system

2.

-ATP

3.

- NifNE

4.

- NifB-co

5.

- homocitrate

6.

+ VnfX (3.0 nmol)

7.

-VnfH

8.

+ Nif-Apodinitrogenase (0.4nmols)

9.

+ VnfX (3.0 nmol), + Nif-Apodinitrogenase (0.4nmols)

10.

+ NifX (3.0 nmols) + Nif-Apodinitrogenase (0.4nmols)

11.

+ NifX (3.0 nmols)

12.

+ NifX (6.0 nmols)

Phosphorimage of an anoxic native gel of in vitro reactions with purified components. The complete system contained 25 mM Tris HCI pH 7.5, MgATP, sodium dithionite, homocitrate, NifB-co, NifNE (0.036 nmols), VnfH (1 nmol) and "Mo in a final volume of 0.5 ml.

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