Materials and Methods

Bacterial strains used: Azospirillum brasilense strains FP2, Sp7, Cd and 245; A. lipoferum Sp59 and JA25; A. amazonense strains Y2 and Y6; A. irakense and A. halopraeferens. Media and growth conditions: strains of A. lipoferum, A. brasilense, A. iraquense and A. halopraeferens were grown in NFb medium and the strains of A. amazonense in LGI medium. Pulsed-field gel electrophoresis (PFGE) of chromosomal DNA was done using a Gene Navigator pulsed-field system (Pharmacia). The chromosomes of S. cerevisiae and S. pombe were used as molecular mass markers. DNA hybridization: A. brasilense 16S and 23S rDNA genes labeled with 32P were used as probes for DNA hybridization. Restriction endonucleases: the enzymes l-Ceul, Xbal and Spel were used.

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