Materials and Methods

Twenty-six isolates of Rhizobium leguminosarum bv. viceae in addition to twenty-three rhizobiophage isolates were isolated from rhizosphere soil samples and from broad-bean nodules obtained from soils of different locations in Egypt. Isolations were done as described by Vincent (1970) and Adams (1959). The rhizobia isolates were tested against the isolated phages (Radwan 2000). The effect of inoculation with sensitive, lysogenic or resistant isolates of R. leguminosarum bv. viceae on nodulation and nitrogen fixation of Vicia faba plants was also studied using Leonard jars.

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