Material and Methods

One hundred and forty-four seedlings of Lotus japonicus, wt were grown for 42 days in sandculture. The nutrient solution contained N 1.5 mM and P 0.075 mM. After day 35, the P supply was changed to 0.5 mM and 0.0005 mM to half of the plants. At day 42 plants were harvested and frozen in liquid N2. Total RNA was extracted and 3 P labeled ss cDNA was hybridized with filters containing 1580 different clones of a cDNA library (nodule tissue of Lotus japonicus). Six micro array filters per treatment have been processed. Data were normalized with the Haruspex expression software.

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