Material and Methods

Nitrogen-fixing endophytes, isolated from sugarcane (var. ML 318) growing in the field in Fontanar, La Habana, were used for the research. T2 is a strain of G. diazotrophicus, and 9C is an unidentified sugarcane endophyte. Both microorganisms have the capacity to fix nitrogen. Ethylene production and H2 evolution kinetics were measured with by gas chromatography (GC) from Qubit System Inc., in cultures with LGI-P medium (Reis et al. 1994) at 30°C. For Total Nitrogenase Activity (TNA), we replaced the N2 with Ar. The Apparent Nitrogenase Activity (ANA) was determined in N2. All measurements occur at 2% O2.

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