Material and Methods

A. brasilense strains FP2 (wild type) (Pedrosa, Yates 1984), 7611 (glnZ::Q) (de Zamaroczy 1998) and 7611 pJIl (glnZ::i1 carrying the plasmid-borne wild-type A. brasilense glnZ gene TcR ) were grown in liquid NFbHPN medium (Pedrosa, Yates 1984) at 120 rpm and 30°C. Plasmid pJIl was constructed by cloning a DNA fragment containing the A. brasilense glnZ gene into the vector pLAFR3.18. Nitrogenase activity was determined by the acetylene reduction method (Scholhorn, Burns 1966).

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