The development of cereal crops that can supply their own mineral nitrogen requirements has been the goal of many researchers. The discovery of G. diazotrophicus (Cavalcante, Dobereiner 1988) offers new promise on achieving significant levels of N2 fixation in cereal crops. The first step in the process of having G. diazotrophicus fix N2 in cereals would be to understand the environmental conditions that affect the growth and nitrogenase activity of G. diazotrophicus. The objectives of this study were to characterize the effects of sucrose, pH, mineral nitrogen and temperature on the cell growth and nitrogenase activity of G. diazotrophicus on solid media and to determine if the bacterium has hydrogenase uptake (Hup) activity.

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