A major limiting factor for legume growth and symbiotic N2 fixation is phosphorus (P). In extreme cases P deficiency prevents nodulation and symbiotic N2 fixation. The legume Lotus japonicus and its microsymbiont Mesorhizobium loti serve as models to study the mechanisms of the inhibitory effect of low P supply on the symbiosis. The aim of this experiment was to evaluate the difference in gene expression of Lotus japonicus grown under a sufficient and a very low phosphorus supply. This may in turn help elucidate the inhibitory mechanism of phosphorus on symbiotic N2 fixation.

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