Non-nodulation (Nod") mutants are useful tools to understand the mechanism of nodulation in legumes. The chickpea non-nodulation lines ICC435M, ICC4918M, ICC4993M (Rupela 1992) and ICCV2M (O.P. Rupela, unpublished) were developed by pure line selection from their normal nodulating parent lines. Flavonoid synthesis/secretion may not be the limiting factor for nodulation in these Nod" lines (Hopper et al. 1998). To identify the nodulation block in Nod" lines, the present study examined their phenotypes and expression of certain early nodulin genes such as ENOD40, ENOD2 and ENOD12.

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