Identification Of Indole3butyric Acid In Azospirillum Cultures

L.J. Martinez Morales , A. Sánchez Ahedo

'Microbiological Research Center, University Autonomous of Puebla, Puebla, Pue. México

2Mexican Institute of Petroleum, México D.F. México

Azospirillum is a metabolically versatile organism with the ability to associate with the agronomically important plants; it produces several metabolites involved in beneficial effects to the plants; for instance, for the production of phytohormones by the microorganism there is more than one pathway for the synthesis of indole-3-acetic acid, however, there has not been described yet a metabolic pathway for the biosynthesis of indole-3-butyric acid by bacteria.

In this work, we show the presence of a substance with auxine activity identified as indole-3-butyric acid. Azospirillum brasílense, was grown at 30°C in a minimal medium for 72 hours and indoles were extracted from the supernatant. This sample was analyzed by high performance liquid chromatography (HPLC), gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The retention times matched those of the authentic IBA standard.

Large scale production of IBA was achieved in cultures grown in fermenters. Identification of the compound was carried out by HPLC and indole butyric acid was collected from the HPLC to perform assays on roots of maize seedlings (Zea mays), inducing the formation of root hair proliferation as well as adventitious roots, the positive biologic activity was observed when compared with authentic IBA standard. It must be noted that this is a report for the actual production of IBA by Azospirillum brasílense.

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