Safety Assessment Under Greenhouse Conditions

H39 and H75 were grown for safety evaluation experiments in a contained greenhouse (Stage I) and in a semi-contained greenhouse (Stage II). These experiments were conducted according to the guidelines established by the Science and Technology Agency of Japan. No significant differences were observed between transformants and wild type plants for reproductive traits, although there were slight differences in plant shape, possibly due to somaclonal variation. No specific peak was observed in gas chromatographic or HPLC analyses of chemical compounds in leaves of the transformants and in areas surrounding the roots. No appreciable effects on soil microflora were exerted by growing the transgenic plants. No difference was observed in their ability to become weeds, either.

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