References

1. Sonnewald U. Expression of E. coli inorganic pyrophosphatase in transgenic plants alters photoassimilate partitioning. Plant J 1992; 2:571-581

2. Lerchl J, Geigenberger P, Stitt M. et al. Impaired photoassimilate partitioning caused by phloem-specific removal of pyrophosphate can be complemented by a phloem-specific cytosolic yeast-derived invertase in transgenic plants. Plant Cell 1995; 7:259-270

3. Herbers K, Sonnewald U. Manipulating metabolic partitioning in transgenic plants. TIBTECH 1996; 14:198-205

4. Sonnewald U, Hajirezaei MR, Kossmann J et al. Increased potato tuber size resulting from apoplastic expression of a yeast invertase. Nature Biotech 1997; 15:794-797

5. Trethewey RN, Geigenberger P, Riedel K et al. Combined expression of glucoki-nase and invertase in potato tubers leads to a dramatic reduction in starch accumulation and a stimulation of glycoly-sis. Plant Journal 1998; 15:109-118

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Table 15.6. Soluble sugars in untransformed potato (Control) and a transgenic plant expressing artichoke SST and FFT (SST/FFT) in fimol/g fresh weight ± standard deviation

Table 15.6. Soluble sugars in untransformed potato (Control) and a transgenic plant expressing artichoke SST and FFT (SST/FFT) in fimol/g fresh weight ± standard deviation

|imol/g FW

Control

SST/FFT

Glc

3.73 ± 4.4

7.99 ± 5.65

Frc

0.36 ± 0.16

0.4 ± 0.2

Suc

34.2 ± 6.5

28.1 ± 6.2

starch

836 ±167

651 ± 110

fructan

n.d.

41.9 ± 7.09

Starch and fructan content is expressed in umol hexose equivalent/g fresh weight. Glc: glucose; Frc: fructose; Suc: sucrose; n.d.: not detectable.

8. Mueller Roeber B, Sonnewald U, Wilmitzer L. Inhibition of the ADP-glucose pyro-phosphorylase in transgenic potatoes leads to sugar storing tubers and influences tuber formation and expression of tuber storage protein genes. EMBO J 1992; 11:1229-1238

9. Mueller-Roeber B, Kossmann J. Approaches to influence starch quantity and starch quality in transgenic plants. Plant Cell Env 1994; 17:601-613

10. Lorberth R, Ritte G, Willmitzer L et al. Inhibition of a starch-granule-bound protein leads to modified starch and repression of cold sweetening. Nature Biotechnology 1998; 16(5):473-477

11. Hellwege EM, Gritscher D, Willmitzer L et al. Transgenic potato tubers accumulate high levels of 1-kestose and nystose: Functional identification of a sucrose sucrose 1-fructosyltransferase of artichoke (Cynara scolymus) blossom discs. Plant J 1997; 12:1057-1065

12. Hellwege EM, Raap M, Gritscher D et al. Differences in chain length distribution of inulin from Cynara scolymus and Helianthus tuberosus are reflected in a transient plant expression system using the respective 1-FFT cDNAs. FEBS Letters 1998; 427: 25-28.

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