The MATVS is based on a novel concept that oncogenes (ipt or rol genes etc.) of Agrobacterium are used as selectable markers to regenerate transgenic cells and select marker-free transgenic plants.4 Commonly, oncogenes have not been used as the selectable marker gene to transform plants because the resulting transgenic plants exhibit a seriously abnormal phenotype. The MATVS is designed to remove the oncogenes from transgenic plants after a transformation by inserting them into removal elements: maize transposable element Ac;5 yeast site-specific recombination system R/ RS (Figs. 25.1 and 25.2).6
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