Mangosteen1416

Mangosteen (Garcinia mangostana) shoots excised from aseptically germinated seedlings were cultured on vermiculite or agar medium with presence (30 g l-1)/absence of sugar and growth regulator (10 ml l-1 of 2-ip and 1 mg l-1 of IBA) in a culture vessel with low (0.1 h-1) or high (4.4 h-1) number of air exchanges at a PPF of 110 ^mol m-2 s-1 under high CO2 concentration (1300 ^mol mol-1 in the culture room). Fresh and dry mass of the plants on day 30 were not significantly different among the treatments. Addition of the

Table 28.2. Effects of CO2 enhancement and supporting material in vitro on damaged leaf percentage and damaged root percentage 1 week after transplanting ex vitro, as well as effect on shoot length, leaf area, and number of primary roots of Eucalyptus plantlets 4 weeks after transplanting ex vitro8

Table 28.2. Effects of CO2 enhancement and supporting material in vitro on damaged leaf percentage and damaged root percentage 1 week after transplanting ex vitro, as well as effect on shoot length, leaf area, and number of primary roots of Eucalyptus plantlets 4 weeks after transplanting ex vitro8

Shoot

Leaf

No. of

CO2

Supporting

Damaged

Damaged

length

area

primary

condition

material

leaves(%)

roots(%)

(mm)

(cm2)

roots

Non-enriched

Agar

33

50

46

27

2

(400 mol mol-1)

Gelrite

29

50

49

29

2

Plastic net

50

33

69

25

6

Vermiculite

10

14

90

40

7

Enriched

Agar

20

40

53

29

2

(1200 mol

mol-1)

Gelrite

18

35

57

40

3

Plastic net

45

28

91

26

7

Vermiculite

5

0

121

49

LSDp=0.05

5

8

12

6

CO2 condition (C) Supporting material (S) C x S

*, ** Significant at P=0.05 and 0.01, respectively. NS, Nonsignificant at P=0.05

r regulator in the medium increased the number of leaves. Twenty to forty percent of shoots exhibited root induction in the treatments with high number of air exchanges and vermiculite as supporting material, either with or without sugar/growth regulator (Fig. 28.5). On the other hand, in the control treatment with low number of air exchanges and sugar-containing agar medium, no rooting was observed (Table 28.6). The CO2 concentration was the lowest, and the net photosynthetic rate per leaf area in the control treatment was about 10 % of that in the treatments with high number of air exchanges (data not shown).

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