Limitation of Use of Small Culture Vessels with High Number of Air Exchanges

As described above, the CO2 concentration inside the culture vessel can be increased and the relative humidity and ethylene concentration can be decreased by attaching gas permeable filter discs to the lid or the wall of the culture vessels, or by changing the gaseous concentrations in the culture room. However, the CO2 concentration and other gaseous concentrations in the culture vessel with natural ventilation are interrelated with a number of factors such as the metabolic activity of the plants in vitro, the size and leaf area of the plants, the number of air exchanges of the culture vessel and the culture room environment. Thus, the gaseous concentrations in the culture vessel with natural ventilation are often unpredictable and uncontrollable. It is also difficult to measure the gaseous concentrations in the vessel continuously. Furthermore, it is difficult to provide a high number of air exchanges for a large culture vessel.

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