We introduce our improvement of a new transformation method (MATVS: MultiAuto-Transformation Vector System) for a practical use. In current systems, selective agents (antibiotics, herbicides etc.) and the corresponding resistance genes (selectable marker genes) are used for the selection of transgenic cells. However, these systems have three problems:
1. The selective agents have negative effects on proliferation and differentiation of plant cells;
2. There is uncertainty regarding the environmental impact of many selectable marker genes;
3. It is difficult to repeat transformation using the same selectable marker in order to pyramid desirable genes.1
The MATVS is designed to overcome these difficulties.2-4
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