Eucalyptus811

Eucalyptus camaldulensisshoots (2.2 cm long) were cultured in vitro photoautotrophi-cally in Magenta type culture vessels (air volume: 370 ml) containing different types of supporting materials (agar, Gelrite, plastic net or vermiculite) for six weeks under CO2 non-enrichment (400 ^mol mol-1 in the culture room) or CO2 enriched (1200 ^mol mol-1 in the culture room) conditions. The gas permeable filter discs (Milli Seal, Millipore Seal, Millipore Japan, Tokyo) were attached to side walls of the vessel. CO2 enrichment significantly increased growth (dry mass and number of primary roots) of plants in vitro regardless of the type of supporting materials (Tables 28.1). The growth in vitro was greatest in the vermiculite, followed by the plastic net, Gelrite, and agar (in descending order) under either CO2-nonenriched or CO2-enriched

Table 28.1. Effects of CO2 enrichment and supporting material in vitro dry mass, leaf area, number of primary roots, and length of primary roots of Eucalyptus plants on 6 weeks in vitro8

CO2 Supporting Dry mass condition material mass (mg)

Non-enriched Agar 45 (400 pmol mol-1)

Gelrite 49

Plastic net 64

Vermiculite 82

Leaf No. of Length of area (cm2) primary primary roots roots(mm)

11 4 39

12 5 42

Enriched Agar (1200 pmol mol-1)

54

9

2

32

Gelrite

62

10

2

37

Plastic net

76

12

5

45

Vermiculite

103

13

6

49

LSDp=0.05

8

3

1

11

Analysis of variance

condition (C)

**

NS

*

*

Supporting material (S)

**

*

**

**

C x S

NS

NS

NS

NS

*, ** Significant at P=0.05 and 0.01, respectively. NS, Nonsignificant at P=0.05.

*, ** Significant at P=0.05 and 0.01, respectively. NS, Nonsignificant at P=0.05.

conditions (Fig. 28.1). The growth of plants ex vitro was highest, and percent damaged leaves/roots was lowest, in the vermiculite under the CO2-enriched condition (Table 28.2). An extensive root system with many secondary roots was produced in vitro in the vermiculite (Fig. 28.2)

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