Cryopreservation

Cryopreservation is at present considered essential for the large scale commercial use of somatic embryogenesis technology for clonal forestry, because it permits genetic material to be saved at low cost until its genetic value can be measured in field tests (see implementation section below). We and others have found that almost 100% of embryogenic culture lines, frozen under controlled conditions and stored in liquid nitrogen, can be recovered after 5 years.6 Trees produced from them are visually indistinguishable from the products of unstored cultures, and there is no evidence of genetic change.6 Space requirements for cryogenic storage are minimal, since even a 45 liter storage unit typically can accommodate 8-10 thousand vials. A completely reliable system for maintenance of liquid nitrogen service, complete with alarm systems and backups, is essential. We have cryostored ESM of over 750

Fig. 29.1. Cotyledonary embryo development of Douglas fir on the pad in a bioreactor: Temporary immersion of the lower pad surface.

Fig. 29.1. Cotyledonary embryo development of Douglas fir on the pad in a bioreactor: Temporary immersion of the lower pad surface.

clones of Douglas fir from superior full-sib families.

Was this article helpful?

0 0
Organic Gardeners Composting

Organic Gardeners Composting

Have you always wanted to grow your own vegetables but didn't know what to do? Here are the best tips on how to become a true and envied organic gardner.

Get My Free Ebook


Post a comment