Construction of cDNA Library in Acacia Mangium

Etiolated two week old seedlings were used. These were grown at 25°C in the dark. Before extraction of all RNA, seedlings were treated with 1 mM of Fe-EDTA solution for 12 h. The extraction of total RNA was performed as described by Brawermam2 and Watanabe and Price.3 Poly (A)+ RNA was purified by an oligo-(dT)-cellulose column. The cDNA was synthesized by using a great length TM cDNA Synthesis Kit (Clontech). The cDNA library was constructed with Agt10, EcoRI, and CIAP-treated Vector Kit (Stratagene).

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