Coffee1213

Growth of Coffea arabusta plants cultured in vitro as affected by sucrose concentration, type of supporting materials and vessel ventilation rate per plantlet was investigated at PPF (photosynthetic photon flux) of 100-200 |mol m-2 s-1. Single nodal cuttings of in vitro coffee plants were cultured on medium without sugar and with 20 g l-1 sucrose. Two types of supporting materials, agar and Florialite (porous solid cubes consisting of a mixture of vermiculite and cellulose fibers, Nisshinbo Ind. Inc., Japan), and two levels of ventilaton rate per plantlet, 27 and 310 ml h-1, were examined (Table 28.3). Fresh mass, shoot length, root length and leaf area of plants cultured on Florialite with sugar-free nutrient solution under high vessel ventilation rate per plantlet were greater than those cultured on sugar-containing agar or Florialite (Fig. 28.3 and

Fig. 28.1. Eucalyptus camaldulensis plantlets cultured for six weeks on sugar-free MS medium under CO2 non-enriched and CO2 enriched conditions, using four different supporting materials (By courtesy of C. Kirdmanee).8 Upper: CO2 non-enriched, Lower CO2 enriched. From left to right: Agar, Gelrite, platic net and vermiculite.

Fig. 28.1. Eucalyptus camaldulensis plantlets cultured for six weeks on sugar-free MS medium under CO2 non-enriched and CO2 enriched conditions, using four different supporting materials (By courtesy of C. Kirdmanee).8 Upper: CO2 non-enriched, Lower CO2 enriched. From left to right: Agar, Gelrite, platic net and vermiculite.

Table 28.4). At the end of a 40 day culture period, callus were observed at the shoot base of plants grown on agar containing sugar. The net photosynthetic rate of coffee plants in vitro was significantly increased when cultured on Florialite with sugar-free nutrient solution under high vessel ventilation rate per plantlet (data not shown).

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