Isolation of Pure Cultures with High Selfaggregation Ability

Eleven pure cultures, distinguished by size, color, and shape of colonies, were isolated from the enrichment culture by plate-spreading technique. Two strains, B and F, with highest aggregation ability were selected from these 11 strains. After that, the sequences of 16S rRNA of these two selected strains were used for identification of species. Microbial strains

Isolation Pure Cultures

Time (days)

Fig. 10.5. Dynamics of aggregation index and cell biomass during batch growth of enrichment culture. (A): Aggregation Index; (■): Turbidity of microbial suspension.

Time (days)

Fig. 10.5. Dynamics of aggregation index and cell biomass during batch growth of enrichment culture. (A): Aggregation Index; (■): Turbidity of microbial suspension.

B and F had AI of 65 and 51%, respectively. Strains B and F can also be coaggregated with each other with coaggregation index of 58%. Cells of both strains were gram-negative aerobic rods.

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