Addition of previously grown microbial biomass as starter culture is a common approach in wastewater treatment plants to start up the conventional activated sludge and anaerobic digestion processes after technological accidents or process failure. Therefore, similar approach can be used to start up the process with granulated microbial biomass. There are some known commercial application of the granules as the seeds to upgrade or initiate wastewater treatment, for example, product ARGUS® (EcoEngineering Ltd., Nova Vas, Croatia) consisting of granules used as the seeds for biological treatment of wastewater from chemical, pharmaceutical, and food industries, as well as oil refineries, landfills, pig, and poultry farms in cases that existing treatment plant is not working properly and should be upgraded and new plant for treatment of complex and toxic chemicals such as phenols, antibiotics, lignosulfonates, naphthalene, and high concentration of nitrogen compounds. The granules have high settling velocity and contain various strains of microorganisms, which are able to accept toxic shocks and perform different physiological functions useful for wastewater treatment. There are no data on the stability of inoculated granules in the conventional wastewater treatment system. Though, it would be reasonable to expect that the inoculated granules will be replaced by more dispersed aggregates after some time of cultivation if there will be no special selection and retention of the granules during wastewater treatment.
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